Transport of hazardous substances is prevalent in road transport, not just in the Republic of Croatia, but beyond. Hazardous substances by nature represent a danger to public safety and therefore must be carefully prepared for transport from the production site to the place of use for the safety of human life and environment protection. As hazardous substances are an unavoidable raw material in industry , an energy generating product, and therefore an indispensable part of everyday life, transport of hazardous substances is the subject of research from a geopolitical, economic, ecological and safety point of view. It is crucial that all work processes are carried out in accordance with the regulations, especially the proper marking of vehicles for the transport of hazardous substances, as this provides information to all traffic participants. The transport od hazardous substance should be given special attention, the properties and characteristics od hazardous substances should be carefully studied, means of transport should be selected and persons in direct contact with hazardous substances should be educated. In order to increase transport security, all participants in the process should receive quality training in order to better understand the dangers which stem from the transport of hazardous substances. The aim of this paper is to warn of the complexity of hazardous substance transport encountered on a daily basis in road transport.