Introduction: Physical activity is associated with many health benefits and in the prevention of chronic non - communicable diseases. Motivation is required to perform a particular physical activity. The primary aim of this research was to determine the level of physical activity of physiotherapy students and motivational factors that influence it. Methods: The study was conducted from February to April 2019 through a questionnaire designed for physiotherapy students on a sample of 270 students. Results: A positive correlation between total physical activity and individual motivational variables was found (stress management p=0,013, refreshment p=0,001, enjoyment of exercise p=0,000, challenge p=0,001, belonging to the group p=0,009, competition p=0,000, strength and endurance p=0,022). A difference in gender and level of physical activity was also found (high intensity activity at work p=0,000, moderate activity at work p=0,041, walking at work p=0,028, total physical activity at work p=0,006). A difference was found in gender of students and motives for exercise (challenge p=0,04, belonging to the group p=0,01, social recognition p=0,02, competition p=0,00). Discussion: According to the data, there are slight differences between men and women in the level of physical activity, however, there is a significant difference in activity at work. Also slight gender differences in exercise motives were found. Conclusion: Further researches are needed to identify the motivating factors for increasing students' physical activity levels so that different programs for the prevention of the negative effects of physical activity can be planned.