Football is one of the most popular sports games in the world. In terms of a physiological classification, football is both an aerobic-anaerobic sport where mixed energetic processes dominate. Football is a complex agonistic kinesiology activity that belongs to a group of multi-structural acyclic movements, and it is characterised by a variability of motor actions to exercise the game and by which a player fulfils the basic objectives of the game: scoring a goal and winning. A football team is made up of eleven players of which one is a goalkeeper, and the others are defined as defenders, middle-fielders, and attackers dependant on the kind of activity and the position on the field. Depending on their position on the field as well as the tasks to perform, the players of a football team can be additionally divided into centre and wing-backs, middle-fielders and forwarders. On these grounds, there occur different requirements to be met by the players with a view to their technical, tactical, morphological, functional, motor as well as psychological performance. The football-related requirements can be measured diagnostically in large part. The sports diagnostics is a group of procedures designated to measure, estimate, and evaluate the fitness of sportsmen. The selected tests and protocols have to be based on a precisely defined testing procedure and adequate metric characteristics. A diagnostic assessment in football encompasses a whole range of the procedures aimed at determination of the football player’s individual characteristics; thus, estimation of the athlete’s overall status. It is often based on the physical fitness, technique, and sporadically, on the situational level. The aim is to find those parameters that best reflect the football player’s ability to achieve the best possible results. The physical fitness factors to be tested in football are as follows: start speed, acceleration, speed endurance, ability to sprint in short intervals, as well as acyclic aerobic-anaerobic endurance.