Introduction: Physical activity is considered a cure for many diseases and conditions. It is even recommended by the World Health Organization, as an exceptional remedy for today’s chronic non-communicable diseases. But in order for it to be implemented, motivation is needed. Motivation drives us to accomplish a goal, and it ultimately leads to improvement. The aim of this paper is to examine the physical activity and level of motivation in students of the Agricultural Forestry School in Vinkovci and the of Economic School in Vukovar. Methods: The research was conducted in the period from April to June 2019 through a survey questionnaire, on a sample of 90 respondents. High school seniors participated. Results: Male students, compared to female students, have a higher overall level of physical activity (MAX = 2040 (M) versus MAX = 1275 (F)). Students of the Agricultural School have a higher level of activity at work, than students of the School of Economics (MAX = 2440 vs. MAX = 1445). All sources of motivation are directed towards good health. Students of the Agricultural School show a higher level of motivation. Male students are also more physically active than female students. Female students, on the other hand, cite stress management and refreshment as the main motives for exercising, while male students emphasize good health, refreshment and dexterity as the most important motives when exercising. Discussion and conclusion: Given the difference in activity by gender, it is concluded that female students are physically more inactive than male students. It is important to determine the reason for this, and how to encourage young people to engage in physical activity, regardless of which high school they attend.